Brahma regulates a specific trans-splicing event at themod(mdg4)locus ofDrosophila melanogaster
نویسندگان
چکیده
منابع مشابه
Viral RNA Trans-Splicing: Chance Event or Product of Evolution?
One year after the discovery of exons and introns in the adenoviral hexon gene by the teams of Roberts and Sharp [1,2], Walther Gilbert suggested that the employment of different exons of a single gene could lead to the generation of various mRNA isoforms [3], a process that is today known as alternative splicing. Alternative splicing implies a number of distinct mechanisms including exon skipp...
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We have designed ribozymes based on a self-splicing group I intron that can trans-splice exon sequences into a chosen RNA target to create a functional chimeric mRNA and provide a highly specific trigger for gene expression. We have targeted ribozymes against the coat protein mRNA of a widespread plant pathogen, cucumber mosaic virus. The ribozymes were designed to trans-splice the coding seque...
متن کاملEstablishment of a novel in vivo sex-specific splicing assay system to identify a trans-acting factor that negatively regulates splicing of Bombyx mori dsx female exons.
The Bombyx mori homolog of doublesex, Bmdsx, plays an essential role in silkworm sexual development. Exons 3 and 4 of Bmdsx pre-mRNA are specifically excluded in males. To explore how this occurs, we developed a novel in vivo sex-specific splicing assay system using sexually differentiated cultured cells. A series of mutation analyses using a Bmdsx minigene with this in vivo splicing assay syst...
متن کاملSelf-splicing and trans-splicing ribozymes
The Journal of Clinical Investigation | August 2003 | Volume 112 | Number 3 The splicing activity of Group I and Group II introns can be harnessed as a molecular tool that may potentially revise any gene of interest. Although hurdles remain, progress has been made in the development of these ribozymes for therapeutic application. While gene therapy is showing promise in the treatment of some di...
متن کاملTrans-splicing and operons.
About 70% of C. elegans mRNAs are trans-spliced to one of two 22 nucleotide spliced leaders. SL1 is used to trim off the 5' ends of pre-mRNAs and replace them with the SL1 sequence. This processing event is very closely related to cis-splicing, or intron removal. The SL1 sequence is donated by a 100 nt small nuclear ribonucleoprotein particle (snRNP). This snRNP is structurally and functionally...
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ژورنال
عنوان ژورنال: RNA Biology
سال: 2014
ISSN: 1547-6286,1555-8584
DOI: 10.4161/rna.27866